Differential miRNA expression profiling reveals miR-205-3p to be a potential radiosensitizer for low- dose ionizing radiation in DLD-1 cells

Indexación: Scopus.Departamento de Oncología Básico-Clínica, Facultad de Medicina, Universidad de Chile, Santiago, Chile 2Comisión Chilena de Energía Nuclear, Santiago, Chile 3Center for Research and Applications in Plasma Physics and Pulsed Power, P4, Chile 4Departamento de Ciencias Físicas, Universidad Andres Bello, Santiago, Chile 5Centro de Investigación y Tratamiento del Cáncer, Facultad de Medicina, Universidad de Chile, Santiago, Chile 6Current Address: Center of Excellence in Precision Medicine, Pfizer, Chile. on IR responsive modeling. This work was supported by Anillo grant ACT1115 and ACT172101, PIA Program, CONICYT; the Chilean doctoral fellowship 21130246Enhanced radiosensitivity at low doses of ionizing radiation (IR) (0.2 to 0.6 Gy) has been reported in several cell lines. This phenomenon, known as low doses hyperradiosensitivity (LDHRS), appears as an opportunity to decrease toxicity of radiotherapy and to enhance the effects of chemotherapy. However, the effect of low single doses IR on cell death is subtle and the mechanism underlying LDHRS has not been clearly explained, limiting the utility of LDHRS for clinical applications. To understand the mechanisms responsible for cell death induced by low-dose IR, LDHRS was evaluated in DLD-1 human colorectal cancer cells and the expression of 80 microRNAs (miRNAs) was assessed by qPCR array. Our results show that DLD-1 cells display an early DNA damage response and apoptotic cell death when exposed to 0.6 Gy. miRNA expression profiling identified 3 over-expressed (miR-205-3p, miR-1 and miR-133b) and 2 downregulated miRNAs (miR-122-5p, and miR-134-5p) upon exposure to 0.6 Gy. This miRNA profile differed from the one in cells exposed to high-dose IR (12 Gy), supporting a distinct low-dose radiation-induced cell death mechanism. Expression of a mimetic miR- 205-3p, the most overexpressed miRNA in cells exposed to 0.6 Gy, induced apoptotic cell death and, more importantly, increased LDHRS in DLD-1 cells. Thus, we propose miR-205-3p as a potential radiosensitizer to low-dose IR. © Andaur et al.http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25405&path[]=7956

3D hydrodynamic model as a tool for more efficient port management and operations.

Ports have been attempting to increase their competitiveness by enhancing their productivity and operate in a more environmentally friendly way. The Port of Seville is located in the Guadalquivir River in the south of Spain and it is the unique Spanish inland port. The estuary has generated and is still generating conflicts of interests. The access channel to the port is being periodically dredged, the natural course has been anthropologically modified several times, original salt marshes have been transformed to grow rice and approximately one-fourth of the total surface of the estuary is now part of two protected areas, one of them is a UNESCO_MAB Biosphere Reserve. Despite its socio-economic and environmental significance there is a surprising lack of scientific and technical information about the environmental interactions between the port activities and the Guadalquivir estuary stakeholders. A 3D hydrodynamic model has been developed to study the tidal regime, water circulation, temperature and salinity distributions, flooding areas and the sediment dynamics in the estuary. The model output has been validated with in situ current speed, direction, water elevation and also with temperature and salinity measurements. Good agreement between modeled and real measurements have been obtained. Our preliminary results show that the vessel traffic management could be improved by using the tidal elevations and currents calculated by the model in the whole estuary. The interactions among the port activities (mainly due of changes in the sediments dynamics), the watershed management and the saline intrusion evolution will be studied in detail. 3D Hydrodynamic Modelling provide spatially explicit information on the key variables governing the dynamics of estuarine areas. The numerical model is a powerful tool to effectively guide the management and operations of ports located in a complex socio-ecological systems.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Adsorción de compuestos fenólicos sobre carbones activados modificados químicamente: efecto del sustituyente en el anillo aromático en las interacciones carbón activado-adsorbato

Se estudió la adsorción de Acetaminofén (4-Hidroxiacetanilida) y Ácido Salicílico (Ácido 2- Hidroxibenzoico) desde solución acuosa en tres carbones activados con diferente química superficial, se llevó acabo la adsorción de Fenol en los mismos adsorbentes con el objetivo de comparar el efecto del sustituyente en el proceso de adsorción. Se emplearon tres carbones activados que presentan distinto contenido de grupos oxigenados; un carbónactivado oxidado con HNO3 (CAO), un carbón activado granular (CAG) y un carbón reducido (CAR)a 1173 K, con el objetivo de evaluar el efecto de los grupos funcionales oxigenados en la adsorción de los compuestos de estudio, se determinó que el aumento en el grado de oxidación superficial en el carbón activado desfavorece el proceso de adsorción de los tres compuestos. Debido a la relación que existe entre la adsorción y las interacciones adsorbato-adsorbente se realizó un estudio calorimétrico para estudiar los cambios energéticos entre los solutos y el carbón activado CAR, que corresponde al carbón activado con mayor capacidad de adsorción para el Acetaminofén, Ácido Salicílico y Fenol

Contribution enthalpic in the interaction of activated carbon with polar and apolar solvents

AbstractA method is presented for calculating the contribution that enthalpies make for every component of mixtures of activated carbon–water and activated carbon–hexane to the immersion enthalpy using the concepts that are used in the solution enthalpies. The immersion enthalpies of microporous activated carbon in water and in hexane have values from −18.97 to −27.21 and −25.23 to −47.89Jg−1, respectively. From the immersion enthalpies and mass relation of the activated carbon in each of the solvents, the differential enthalpies are calculated for the activated carbon in water, HwDIFac, with values between −15.95 and −26.81Jg−1, as are the differential enthalpies for the activated carbon in hexane, ΔHhDIFac, with values between −6.86 and −46.97Jg−1. For a low mass relation of the mixture components the contributions to the immersion enthalpy of the activated carbon and water differ by 3.20Jg−1, while the difference between the contributions of the activated carbon and hexane is 19.41Jg−1

Integration of intermittent measurement from in-cylinder pressure resonance in a multi-sensor mass flow estimator

[EN] A novel technique of trapped mass determination, based on the in-cylinder pressure resonance, has been recently published by the authors. However, the method only works when sufficient resonance intensity exists and the current formulation might preclude its implementation in real-time due to excessive computational burden. The present paper proposes an iterative algorithm for reducing the number of operations, an adaptive filter to identify faulty measurements and a Kalman filter that combines several sensors and models, currently used in commercial light-duty engines, to ensure a continous estimation of trapped mass, air mass, and exhaust gas recirculation (EGR). The filter is implemented using experimental data of a EURO 6 light-duty engine in a world harmonize light-duty test cycle (WLTC), showing the potential of being implemented in real driving conditions with robustness and harnessing a new measurement to improve the accuracy and response of current estimations.Guardiola, C.; Pla Moreno, B.; Bares-Moreno, P.; Peyton Jones, J. (2019). Integration of intermittent measurement from in-cylinder pressure resonance in a multi-sensor mass flow estimator. Mechanical Systems and Signal Processing. 131:152-165. https://doi.org/10.1016/j.ymssp.2019.05.052S15216513

Erythemal ultraviolet exposure in two groups of outdoor workers in Valencia, Spain

[EN] UV exposure is considered to be one of the most important risk factors in skin cancers, mainly in outdoor occupational activities. Outdoor workers receive regular and significant solar UV erythemal radiation (UVER). To quantify the UVER exposure of certain groups of workers, dosimeters are used to measure the biologically effective UV radiation received in the course of their daily work. Two groups of outdoor workers, composed of gardeners and lifeguards, were measured for UVER exposure using sensitive spore-film filter-type personal dosimeters (Viospor). The study took place in Valencia, Spain, in June and July 2008, and involved one group of four gardeners and another of five beach lifeguards for a period of 4 and 6 days, respectively. The gardeners' mean UV exposure was 4.13 +/- 0.60 SED day(-1), where 1 SED is defined as effective 100 J m(-2) when weighted with the CIE erythemal response function, whereas the lifeguards received 11.43 +/- 2.15 SED day(-1). The mean exposure ratio (ER) relative to ambient of gardeners was 0.09 +/- 0.01 and for lifeguards was 0.27 +/- 0.05. ER is defined as the ratio between the personal dose on a selected anatomical site and the corresponding ambient dose on a horizontal plane during the same exposure period. The lifeguards received the highest UVER exposure, although both groups had measured UVER exposure in excess of occupational guidelines, indicating that protective measures are necessary.This research was supported by the Spanish Ministry of Education and Science within the research project CGL2007-61813. The authors thank the gardening staff of the UPV and the lifeguards and staff of the Red Cross for their cooperation in this study. We also thank the State Agency for Meteorology and the Generalitat Valenciana for providing us with access to their meteorological data. We finally thank the R&D&D&I Linguistic Assistance Office, Universidad Politecnica de Valencia (Spain), for granting financial support for the linguistic revision of this paper.Serrano, MA.; Cañada, J.; Moreno, J. (2009). Erythemal ultraviolet exposure in two groups of outdoor workers in Valencia, Spain. Photochemistry and Photobiology. 85(6):1468-1473. https://doi.org/10.1111/j.1751-1097.2009.00609.x1468147385

Erythemal Ultraviolet Exposure of Cyclists in Valencia, Spain

[EN] UV exposure is considered to be one of the most important risk factors in skin cancers, mainly in outdoor occupational or recreational activities. Outdoor athletes such as cyclists receive regular and significant solar UV erythemal radiation (UVER). The aim of this work was to quantify UVER exposure of amateur cyclists over the course of several days in their training schedules. To quantify UVER exposure of this group, dosimeters (Viospor) were attached at the top of the helmet in the course of their training. The study took place in Valencia, Spain, in June to July 2008 and February to March 2009, and involved a group of five cyclists over a period of 4 days for each period. The mean 2-day personal UV exposure was 32.24 +/- 4.14 SED (standard erythema dose) in summer and 11.30 +/- 5.36 SED in the winter period. One SED is defined as an effective radiant exposure of 100 J m-2 when weighted with the International Commission on Illumination (CIE) erythemal response function. The mean exposure ratio (ER) of cyclists was 0.37 +/- 0.04 in summer and 0.40 +/- 0.11 in winter. The cyclists received the highest UVER exposure in the summer period, but in both training periods UVER exposure was in excess of occupational and recreational guidelines, indicating that protective measures are very necessary.The authors thank the members of the UPV Cycle Club for their cooperation in this study. We also thank the State Agency for Meteorology and the Generalitat Valenciana for providing us with access to their meteorological data. We would like to thank the R&D and innovation Linguistic Assistance Office, Universidad Politecnica de Valencia (Spain), for granting financial support for the linguistic revision of this paper. The research reported here was supported by the Spanish Ministry of Education and Science within the research project CGL2007-61813.Serrano, M.; Cañada, J.; Moreno, J. (2010). Erythemal Ultraviolet Exposure of Cyclists in Valencia, Spain. Photochemistry and Photobiology. 86(3):716-721. https://doi.org/10.1111/j.1751-1097.2009.00693.xS71672186

Affine convex body semigroups

In this paper we present a new kind of semigroups called convex body semigroups which are generated by convex bodies of R^k. They generalize to arbitrary dimension the concept of proportionally modular numerical semigroup of [7]. Several properties of these semigroups are proven. Affine convex body semigroups obtained from circles and polygons of R^2 are characterized. The algorithms for computing minimal system of generators of these semigroups are given. We provide the implementation of some of them